Antigen-specific proliferation of murine lymph node cells in vitro.

Autor: Palaszynski, Susan, Palaszynski, Edmund, Noonan, Frances
Zdroj: Journal of Tissue Culture Methods; 1992, Vol. 14 Issue 1, p21-24, 4p
Abstrakt: A method is described for determining the antigen-specific proliferative response of murine cells to foreign antigens in vitro. Methods are given for immunizing mice and for preparing lymphoid cells. Culture of lymphoid cells with antigen and quantitation of the proliferative response by determination of the uptake of tritiated thymidine is described. Applications of this methodology are indicated. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index