| Autor: |
Kishimoto, Takuji, Oguri, Tetsuhisa, Ueda, Daizo, Tada, Manabu |
| Zdroj: |
Archives of Toxicology; Oct1995, Vol. 69 Issue 10, p718-721, 4p |
| Abstrakt: |
The effect of lead acetate (Pb) on the growth of and tube formation by cultured human umbilical vascular endothelial cells (HUVEC) was examined. HUVEC were collected by enzymatic digestion with collagenase. The number of viable cells of HUVEC was negligibly affected by cultivation with Pb at concentrations of 1-100 μM, but was slightly reduced by cultivation at 500 μM. Tube formation was studied by culturing the cells on a gelled basement membranen matrix (Matrigel). Treatment of HUVEC with 0.1-50.0 μM Pb for 24 h inhibited tube formation dose-dependently. The length of tube formation decreased time-dependently with 1.0 μM Pb. These findings suggest that Pb inhibits the formation of a capillary network by HUVEC, and that Pb could be injurious to endothelials cell function. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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