| Autor: |
Felley-Bosco, E., Diezi, J. |
| Zdroj: |
Archives of Toxicology; Feb1991, Vol. 65 Issue 2, p160-163, 4p |
| Abstrakt: |
LLC-PK cells, an established cell line from pig kidney with proximal tubule properties, were cultivated in vitro at confluence on plastic dishes. They were then exposed (apical side) to inorganic cadmium (CdCl, 5 μM) for periods ranging between 1 to 24 h. Analysis of the cell supernatant after homogenisation and ultracentrifugation indicated that Cd taken up in the first 3 h was bound to cytosolic high molecular weight proteins, but was redistributed to low molecular weight proteins at later stages. Induction of Cd-metallothionein (Cd-Mt) synthesis, as judged from Cd-Mt binding to a specific anti-Cd-Mt antibody and from the rate ofS-cys incorporation into a specific protein fraction, was apparent 3-6 h after the addition of Cd to the incubation medium. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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