| Autor: |
Kishimoto, Takuji, Fukuzawa, Yoichiro, Tada, Manabu |
| Zdroj: |
Archives of Toxicology; Jul1990, Vol. 64 Issue 5, p383-386, 4p |
| Abstrakt: |
The combined effect of cadmium (CdCl) and high temperature on the subsequent growth, the synthesis of nucleic acids and protein, and the cell cycle in HeLa S cells was investigated. The subsequent growth rate was depressed by cadmium at 37.1°C. This suppression effect was enhanced at 40.4° C compared with the effect at 37.1° C. The synthesis of DNA, RNA and protein was inhibited at higher temperatures in cultivation without cadmium, and depressed at each temperature in a concentration-dependent manner by the addition of cadmium. The DNA, RNA and protein-IC values at 40.4° C decreased compared with the values at 37.1° C. The DNA and RNA-IC values were significantly decreased ( p<0.01, p<0.005, respectively) depending on the temperature. After treating the cells with cadmium at 40.4° C, the DNA/BrdUrd distribution showed that the rate of dead cells increased and the rate of the G/G phase decreased. These results indicate that the inhibitory effect of cadmium on the subsequent growth of HeLa S cells is enhanced at high temperature and this enhancement is related to the increased inhibitory effect of cadmium on DNA and RNA synthesis at high temperature. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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