Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA.

Autor: Fujita, Masaki, Morooka, Tatsuya, Fujimoto, Shuji, Moriya, Tetsuhiro, Amako, Kazunobu
Zdroj: Archives of Microbiology; Dec1995, Vol. 164 Issue 6, p444-447, 4p
Abstrakt: Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index