Immunological recognition of specific antigenic determinants in UV- and gamma-irradiated phage DNA.

Autor: Hotz, G.
Zdroj: Radiation & Environmental Biophysics; Mar1975, Vol. 12 Issue 1, p41-50, 10p
Abstrakt: Thermally denatured DNA of coliphage T1 after treatment with uv-light (2537 Å) andCo-gamma rays acts as a hapten with antigenic determinant groups specific for radiation-induced alterations of the macromolecule. After conjugation to methylated bovine serum albumin the DNA becomes immunogenic in rabbits. Antibodies against irradiated DNA do not react with unirradiated single-stranded DNA. Antigen-antibody complexes were demonstrated by CsCl-density gradient centrifugation. The decrease in buoyant density of the DNA is proportional to the amount of antibody protein bound to the antigen. By this means photoproducts as well as alterations due to ionizing radiation in DNA were detected independent of the type of antigen-antibody complex, i.e. precipitating or soluble aggregate. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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