| Autor: |
Farkas, B., Bonnekoh, B., Mahrle, G. |
| Zdroj: |
Archives of Dermatological Research; Jul1991, Vol. 283 Issue 5, p337-341, 5p |
| Abstrakt: |
The hyperproliferative human keratinocyte line (HaCaT) was tested for dithranol tolerance (tachyphylaxis) at the cellular level. At day 4 after seeding, keratinocytes were treated with 0.3 or 1.0 Μ M dithranol. Data were compared with those from experiments including additional pretreatments with 0.3 Μ M at day 3, or at days 1 and 3. Protein content, DNA synthesis and protein synthesis (incorporation of H-thymidine and C-amino acids per protein) were determined at 24, 48 and 72 h after the last drug exposure. Protein content of attached cells decreased in relation to dose and frequency of treatments. Inhibition of DNA and protein synthesis (38.2% and 32.3%, respectively) also occurred 24 h after a single treatment with 0.3 Μ M dithranol, but was only 18.4% and 9.1% after pretreatment twice with 0.3 Μ M dithranol. This tolerance reaction in vitro, after repeated dithranol exposure of human keratinocytes, may be explained by a selective loss of drug-sensitive cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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