| Autor: |
Ito, Hiroyuki, Nakajima, Toshiaki, Takikawa, Reiko, Hamada, Eiji, Iguchi, Mari, Sugimoto, Tsuneaki, Kurachi, Yoshihisa |
| Zdroj: |
Naunyn-Schmiedeberg's Archives of Pharmacology; 1991, Vol. 344 Issue 1, p133-136, 4p |
| Abstrakt: |
The effect of coenzyme Q (CoQ) on the cyanide (CN)-induced ATP-sensitive K channel current (K) was examined in single atrial myocytes, using the patch clamp technique. Superfusion of the cells with a CN/low glucose bathing solution induced an outward current in the whole-cell clamp condition. Glibenclamide (1 μM) abolished this current, indicating that the current was carried through the K channel. After steady-state activation by CN, pinacidil (a K channel opener, 300 μM) failed to further increase the current. In cell-attached patches, CN, when applied to the bath, induced bursting openings of an 80 pS channel (the K channel). In cells preincubated for 30 min in a solution containing CoQ (100 μg/ml), CN-activation of the K channel was markedly attenuated both at the whole cell and at the single channel level. At the steady-state effect of CN in CoQ-treated cells, pinacidil (300 μM) activated the current to the maximum level achieved by CN in the control cells. These results suggest that CoQ reduces in the CN-induced KATP current not by affecting the channel itself but by preventing depletion of intracellular ATP caused by CN. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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