A rapid and simple procedure for the determination of guanosine 3′,5′-monophosphate by use of the protein-binding method.

Autor: Dinnendahl, V.
Zdroj: Naunyn-Schmiedeberg's Archives of Pharmacology; 1974, Vol. 284 Issue 1, p55-61, 7p
Abstrakt: A modification of the protein-binding assay for guanosine 3′,5′-monophosphate (Murad et al., 1971) is decribed yielding a substantial increase in sensitivity together with a more simplified procedure. The method employs a purified enzyme preparation from lobster tail muscle with a dissociation constant for cGMP of about 5 nM. The nucleotide-protein complex is separated from free cGMP by charcoal treatment. 0.05-0.1pmoles cGMP are detectable by this procedure. cGMP contents of seven discrete regions of rat brain were determined to show the usefullness of the described method. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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