Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain.

Autor: Liposits, Zsolt, Görcs, Tamás, Sétáló, György, Lengvári, István, Flerkó, Béla, Vigh, Sándor, Schally, Andrew
Zdroj: Cell & Tissue Research; 1983, Vol. 229 Issue 1, p191-196, 6p
Abstrakt: The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80-120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index