Immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants.

Autor: Ravelonandro, M., Peyruchaud, O., Garrigue, L., Marcillac, G., Dunez, J.
Zdroj: Archives of Virology; 1993, Vol. 130 Issue 3/4, p251-268, 18p
Abstrakt: Tobacco plants ( Nicotiana tabacum cv. Xanthi) have been transformed via Agrobacterium tumefaciens vectors, with cDNAs corresponding to the plum pox virus (PPV) cistron 2 encoding helper component (HC-Pro) and with the first two and half cistrons of the PPV genome. Presence of the HC-Pro in PPV-infected plants and transgenic plants transformed with the gene coding for this protein was investigated using specific polyclonal antibodies produced against the PPV HC-Pro. The results suggest that two proteases are involved in the processing of the PPV N-terminal polyprotein to yield a protein of 48 k (HC-Pro). HC-Pro autolytically cleaves at its carboxyl-terminus and a proteolytic activity, probably associated with the protein (P 1) encoded by the cistron 1, is required for the cleavage in planta between the proteins derived from cistrons 1 and 2. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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