Synthetic peptides corresponding to the F protein of RSV stimulate murine B and T cells but fail to confer protection.

Autor: Trudel, M., Stott, E., Taylor, G., Oth, D., Mercier, G., Nadon, F., Séguin, C., Simard, C., Lacroix, M.
Zdroj: Archives of Virology; 1991, Vol. 117 Issue 1/2, p59-71, 13p
Abstrakt: We have previously located a major neutralization site of the fusion protein of respiratory syncytial virus (RSV) in the polypeptide region extending from amino acids Ile221 to Glu232. In this report, 8 peptides corresponding to the six major hydrophilic regions of the F subunit were selected to analyse their immunogenic and protective capacities as well as their ability to block the high neutralization activities of 4 monoclonal antibodies (MAbs). Only 5 of the 8 peptides tested induced specific antibodies while all induced an in vitro interleukin-2 response of splenocytes from immunized mice. Peptide 3 (Ile221-Phe237) was able to elicit neutralizing antibodies, confirming our previous hypothesis concerning the location of a neutralization site. However, immunization with the latter did not induce significant reduction of virus in lungs of BALB/c mice upon challenge, probably due to an inadequate level of circulating neutralizing antibodies. Interestingly, peptides 2 (Asn216-Glu232), 3 (Ile221-Phe237), and 5 (Ser275-Ile288) blocked in vitro neutralization by four different F specific MAbs. A hypothesis is proposed to explain these results. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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