| Autor: |
Cohen-Haguenauer, Odile, Barton, Paul, Cong, Nguyen, Cohen, Arlette, Masset, Michel, Buckingham, Margaret, Frézal, Jean |
| Zdroj: |
Human Genetics; 1989, Vol. 81 Issue 3, p278-282, 5p |
| Abstrakt: |
In all eukaryotes, myosin plays a major role in the maintenance of cell shape and in cellular movement; in association with actin and other contractile proteins it is also a major structural component of the muscle sarcomere. Several isoforms of myosin alkali light chain have been identified, associated with different muscle types. We have recently localized the gene encoding the fast skeletal muscle alkali light-chain isoforms MLC1 and MLC3 (HGM symbol, MYL1) to human chromosome 2q32.1-qter (Cohen-Haguenauer 1988). We present here the chromosomal assignment of two loci encoding the ventricular muscle isoform MLC1 (equivalent to the slow skeletal muscle isoform MLC1) and the atrial muscle isoform MLC1 (equivalent to the fetal isoform MLC1) using a panel of 25 independent man-rodent somatic cell hybrids. The MLC1 gene (HGM symbol, MYL3) was mapped to human chromosome 3 using a human full-length cDNA probe that hybridizes to a single major human TaqI 2.8-kb fragment. The MLC1 probe (HGM symbol, MYL4) was a 360-bp mouse cDNA fragment that gave a distinct signal with human DNA using low stringency conditions of hybridization and washings and after presaturation of the Southern blots with rodent DNA. A single PstI 7.8-kb fragment gives an intense signal, and its presence correlates with the presence of chromosome 17 among the hybrids. These data are in keeping with the localizations of the MLC1 gene to mouse chromosome 9, and of the MLC1 gene to mouse chromosome 11, which share some markers in common with human chromosomes 3 and 17 respectively. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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