| Autor: |
Lodin, Z., Novák, J., Holečková, E., Hartman, J. |
| Zdroj: |
Zeitschrift Für Zellforschung und Mikroskopische Anatomie; 1968, Vol. 85 Issue 2, p158-164, 7p |
| Abstrakt: |
The effect of methionine-sulphoximine (MSI) on the behaviour of the nerve and glia elements from brain and cerebellum of rat and chick in tissue culture was studied. It was observed that a concentration of MSI M inhibits the growth of early brain expiants. Concentrations of 10 and 10 M did not show this effect. Time lapse cinematographic recordings evaluating continually the behaviour of living glial and neuronal elements in tissue culture showed that MSI in a concentration of 10 caused, very shortly after administration, a conspicuous oedema of cellular elements. The highest degree of swelling occurred in the perikarya and processes of astrocytes. Cell bodies of the oligodendroglia swelled only in the beginning and later shrank again considerably. This shrinkage was accompanied by a retraction of the glial processes, their fragmentation, and destruction. The perikaryon of the oligodendroglia contracted discontinually. Similarly the volumetric increase of astrocytes was accompanied by rhythmic contractions. In neuronal as well as oligodendroglial perikarya movements of mitochondria and other cytoplasmic particles first accelerated, later slowed down. Synchronously with cytoplasmic swelling of neurons the formation of many vacuoles is observed. From a comparison of these changes, their time course, and the degree of damage in various cell types it may be concluded that MSI attacks in the first place glial elements, among them most intensely the oligodendroglia. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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