| Abstrakt: |
Yaba poxvirus morphogenesis, from the penetration to the appearance of the progeny virus, was studied in Jinet cells (a cynomolgus monkey kidney cell line). Within 3 hours after infection, the adsorption of virus particles by the cells were observed. In the next, the disruptions of viral surface coat and phagocytic vacuole membrane occurred, and the viral core membrane was finally disrupted. Thus, the DNA-containing viral core substances dispersed in the cytoplasm were recognized as the lower density area, the periphery of which was surrounded by many ribosomal aggregates. The viral uncoating area appeared to correspond to the viral factory to be formed, since the viral protein and DNA synthesis was first detected in such an area at 7 and 9 hours p.i., respectively. A typical viral factory after 24 hours p.i. included the virus particles at various stages of the maturation process including the immature virus particles and virus-related structures such as micelles. The immature virus particle was encircled with a unit membrane which was covered with subunits being arranged vertically to the surface. The immature particle was 3000-4000 A in diameter and the spherical micelle was about 400 A in diameter with a unit membrane of about 70 A thick corresponding to the inner membrane of the immature virus particle. In addition, the formation of immature virus particles with micelles had been observed in Yaba virus-infected Jinet cells in the presence of hydroxyurea, and consequently the Yaba immature virus particles had been formed without the synthesis of progeny viral DNA. After the completion of immature virus particle formation, assembly of new membranous structures appeared inside the virus particle, presumably forming the viral core membrane. The shape of the viral core changed to dumbbell-form making the lateral bodies evident and the mature virus was completed. [ABSTRACT FROM AUTHOR] |
