Isolation of the SV40 induced tumor ('T') antigen from transformed hamster kidney cells.

Autor: Spira, G., Popescu, M., Cymbalista, S., Biezunski, N., Goldblum, N.
Zdroj: Archiv für die Gesamte Virusforschung; 1972, Vol. 37 Issue 2/3, p236-242, 7p
Abstrakt: A procedure for the isolation and partial purification of tumor ('T') antigen induced by SV 40 virus in transformed hamster kidney cells is described. The method involves differential precipitation with ammonium sulfate, fractionation on a hydroxylapatite column and concentration by ultrafiltration. There is very little loss in activity as measured in total CF units and the degree of purification is about one hundredfold. Analysis of the purified preparation indicates four peaks of CF activity ranging in molecular weight from 70,000-400,000. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index