Benzodiazepine receptor recruitment after acute stress in synaptosomal membranes from forebrain of young chicks: action of Triton X-100.

Autor: Martijena, I., Salvatierra, N., Arce, A.
Zdroj: Journal of Neural Transmission; 1992, Vol. 87 Issue 2, p97-104, 8p
Abstrakt: In young chicks submitted to acute stress by forced swimming there was a significant increase in the number of the measurable [ H]-flunitrazepam receptors in synaptosomal membranes from forebrain. In addition, low sub-solubilizing concentrations of Triton X-100 caused a significant increase in the measurable [H]-flunitrazepam receptor number in synaptosomal membranes from non-stressed chicks. However, this Triton X-100 stimulatory effect was not observed when tested in synaptosomal membranes from stressed chicks. In all cases the affinity remained unchanged. This result suggest that: (i) acute stress and Triton X-100 induce receptor recruitment by enhancing [ H]-flunitrazepam accessibility to a pool of receptors which is unmeasurable either before stress or in absence of detergent; (ii) neither recruitment types are additive and they involve receptors coming from the same nonmeasurable pool; (iii) stress induces a maximal recruitment of existing benzodiazepine receptors; (iiii) the pool of nonmeasurable receptors represents about a quarter of the total in control chicks. The recruitment at a short time of stress could be interpreted in terms involving internalization; recycling or modulation of receptors but not its biosynthesis or degradation. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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