| Autor: |
Zanquetta, Melissa Moreira, Correa-Giannella, Maria Lúcia, Giannella-Neto, Daniel, Alonso, Paulino Alberto, Guimarães, Ligia Maria Martins Vaz, Meyer, Alberto, Villares, Sandra Mara Ferreira |
| Předmět: |
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| Zdroj: |
Chronobiology International: The Journal of Biological & Medical Rhythm Research; Apr2012, Vol. 29 Issue 3, p252-260, 9p, 3 Charts, 2 Graphs |
| Abstrakt: |
Disrupted circadian rhythms are associated with obesity and metabolic alterations, but little is known about the participation of peripheral circadian clock machinery in these processes. The aim of the present study was to analyze RNA expression of clock genes in subcutaneous (SAT) and visceral (VAT) adipose tissues of male and female subjects in AM (morning) and PM (afternoon) periods, and its interactions with body mass index (BMI). Ninety-one subjects (41 ± 11 yrs of age) presenting a wide range of BMI (21.4 to 48.6 kg//m2) were included. SAT and VAT biopsies were obtained from patients undergoing abdominal surgeries. Clock genes expressions were evaluated by qRT-PCR. The only clock gene that showed higher expression ( p < .0001) in SAT in comparison to VAT was PER1 of female (372%%) and male (326%%) subjects. Different patterns of expression between the AM and PM periods were observed, in particular REV-ERBα, which was reduced ( p < .05) at the PM period in SAT and VAT of both women and men (women: ∼53%% lower; men: ∼78%% lower), whereas CLOCK expression was not altered. Relationships between clock genes were different in SAT vs. VAT. BMI was negatively correlated with SAT PER1 ( r == −.549; p == .001) and SAT PER2 ( r == −.613; p == .0001) and positively with VAT CLOCK ( r == .541; p == .001) and VAT BMAL1 ( r == .468; p == .007) only in women. These data suggest that the circadian clock machinery of adipose tissue depots differs between female and male subjects, with a sex-specific effect observed for some genes. BMI correlated with clock genes, but at this moment it is not possible to establish the cause-effect relationship. (Author correspondence: ) [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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