An expression system based on the promoter region of the Aspergillus awamori 1,4-β-endoxylanase A gene.

Autor: Gouka, R. J., Hessing, J. G. M., Punt, P. J., Stam, H., Musters, W., den Hondel, C. A. M. J. J. Van
Zdroj: Applied Microbiology & Biotechnology; 1996, Vol. 46 Issue 1, p28-35, 8p
Abstrakt: A new, highly inducible fungal promoter derived from the Aspergillus awamori 1,4-β-endoxylanase A ( exlA) gene is described. Induction analysis, carried out with the wild-type strain in shake flasks, showed that exlA expression is regulated at the transcriptional level. Using a β-glucuronidase ( uidA) reporter strategy, D-xylose was shown to be an efficient inducer of the exlA promoter, whereas sucrose or maltodextrin were not. Upon D-xylose induction, the exlA promoter was threefold more efficient than the frequently used A. niger glucoamylase ( glaA) promoter under maltodextrin induction. Detailed induction analyses demonstrated that induction was dependent on the presence of D-xylose in the medium. Carbon-source-limited chemostat cultures with the uidA reporter strain showed that D-xylose was also a very good inducer in a fermenter, even in the presence of sucrose. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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