| Autor: |
Sasaki, Joji, Miyazaki, Akiko, Saito, Mika, Adachi, Takashi, Mizoue, Kazutoshi, Hanada, Kazunori, Omura, Sadafumi |
| Zdroj: |
Applied Microbiology & Biotechnology; 1992, Vol. 38 Issue 2, p152-157, 6p |
| Abstrakt: |
To enzymatically synthesize active metabolites of vitamin D, we screened about 500 bacterial strains and 450 fungal strains, of which 12 strains were able to convert vitamin D to 1α,25-dihydroxyvitamin D [1α,25(OH)D] via 25-hyroxyvitamin D [25(OH)D]. The conversion activity was only detected in strains belonging to the genus Amycolata among all the organisms tested. A preparative-scale conversion of vitamin D to 25(OH)D and 1α,25(OH)D in a 200-1 tank fermentor using A. autotrophica FERM BP-1573 was accomplished, yielding 8.3 mg 25(OH)D/l culture and 0.17 mg 1α,25(OH)D/l culture. A related compound, vitamin D, could be also converted to 25-hydroxyvitamin D and 1α,25-dihydroxyvitamin D using the same strain. The cytochrome P-450 of FERM BP-1573 was detected by reduced CO difference spectra in whole-cell suspensions. Vitamin D in the culture induced cytochrome P-450 and the conversion activity simultaneously, suggesting that the hydroxylation at C-25 of vitamin D and at C-1 of 25(OH)D originates from cytochrome P-450. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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