| Abstrakt: |
Candida utilis cells were grown in continuous culture in a medium with ammonium or arginine as the nitrogen source. Arginine produced a marked change in the amount of polyphosphates and arginine in whole cells and vacuoles, as well as in the ratio of the concentrations of these substances. The specific growth rate (μ) which in continuous culture is equivalent to the dilution rate (D), affects the amount and chain length of the polyphosphates and also the arginine content of the vacuoles and whole cells. Thus, if D is increased the amount of polyphosphates per milligram protein is increased. There is apparently a direct and linear relationship between D, the specific growth rate (μ) and the polyphosphate content. Changes in D also affect the length of the polyphosphate chain, and the relationship is inverse. At low growth rates, two types of chain were observed, one of approximately 35 phosphate units and the other of 5 units. At high growth rates phosphorus was not stored as longchain polyphosphates. [ABSTRACT FROM AUTHOR] |
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