| Autor: |
Blasig, R., Huth, J., Franke, P., Borneleit, P., Schunck, W., Müller, H. |
| Zdroj: |
Applied Microbiology & Biotechnology; 1989, Vol. 31 Issue 5/6, p571-576, 6p |
| Abstrakt: |
The yeast Candida maltosa precultivated on liquid n-alkanes utilized different solid n-alkanes (especially C−C) in the presence of pristane as an organic phase with rates comparable to, or somewhat larger than, those of liquid n-alkanes. Analysis of cellular fatty acids indicated an assimilation of solid n-alkanes via monoterminal oxidation. The resulting fatty acids with substrate chain length were chain-shortened by C units down to an optimal range of chain length from C to C and incorporated into cellular, lipids directly or after desaturation. The intermediates of chain-shortening with numbers of carbon atoms higher than C, as well as the unusually long-chain fatty acids of substrate chain length, were detected in trace amounts only. Even-carbon-numbered and odd-numbered fatty acids predominated in experiments with evenchain and odd-chain n-alkanes, respectively. Studies with cerulenin indicated that de novo synthesis of fatty acids was negligible. Oxidation of solid n-alkanes by the yeast C. maltosa yielded fatty acid patterns similar to those of cells grown on liquid n-alkanes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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