Regeneration of NAD(H) covalently bound to formate dehydrogenase with several second enzymes.

Autor: Kato, Nobuo, Yamagami, Tomohide, Shimao, Masayuki, Sakazawa, Chikahiro
Zdroj: Applied Microbiology & Biotechnology; 1987, Vol. 25 Issue 5, p415-418, 4p
Abstrakt: N-[ N-(6-Aminohexyl)carbamoylmethyl]-NAD was covalently bound to formate dehydrogenase. The formate dehydrogenase-NAD complex, which contained 0.2 mol of reactive NAD moiety per subunit, functioned as an NAD(H)-regeneration system for a second coupled reaction involving one of the following enzymes; lactate, malate, alanine and leucine dehydrogenases, whose reductive reactions proceeded stoichiometrically in the absence of exogenous NAD. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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