| Autor: |
Nilsson, Inge, Dostálek, Milan |
| Zdroj: |
Applied Microbiology & Biotechnology; 1984, Vol. 20 Issue 3, p183-188, 6p |
| Abstrakt: |
Test systems were set up in order to evaluate the ability of biomass from a continuous culture to form biofilms. A film-forming strain of Pseudomonas putida was used as the test organism. The adsorption of resting cells onto glass surfaces was measured in specially designed chambers containing 1 ml of cell suspension. Both the quantity and the physiological activity of the adsorbed cells, in terms of optical density after detachment and pH change of a substrate exposed to the adsorbed cells, were measured. The analysis of biomass from continuous cultures of Pseudomonas putida verified the suitability of the methods. Furthermore, other properties of importance to biofilm formation such as hydrophobicity and flocculation capacity of the cells were investigated. It was shown for samples deriving from different dilution rates that the cell adsorption rate drastically increased at dilution rates higher than the μ of the culture. Simultaneously, higher values of hydrophobicity and flocculation capacity were observed. It was also shown that the age and thickness of the biofilm subsequently produced in the continuous culture influenced the metabolic activity per unit of biomass attached to the surface. The methods described in this investigation may facilitate the study of parameters important to biofilm formation as well as the metabolic activity of the attached biomass. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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