A specific, sensitive and high-capacity immunoassay for PAF.

Autor: Baldo, Brian, Smal, Mary, McCaskill, Alistair
Zdroj: Lipids; Dec1991, Vol. 26 Issue 12, p1136-1139, 4p
Abstrakt: A specific radioimmunoassay for platelet-activating factor (PAF) sensitive in the range 10-1000 pg (0.02-2 pmoles) has been developed. Detailed quantitative hapten inhibition studies showed specificity for the acetyl group at C-2 of PAF, a requirement for the ether linkage at C-1 and some tolerance for substituents on the choline nitrogen. No significant cross-reactivity was found with phosphatidylcholine and lysophosphatidylcholine or with lysoPAF. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index