| Abstrakt: |
Endogenous phospholipase A (PLA) specific activity (SA) (% hydrolysis/min/mg protein) in the rat pleural cavity, measured as the acyl-hydrolysis of [H]-arachidonic acid E. coli substrate, was quantitated after induction of a reverse passive Arthus reaction (RPAR). PLA SA, derived when the rate of hydrolysis was linear (1-5 min), was 1.9, 1.4, 3.8 and 4.1% h/min/mg at 10 min, 2 h, 4 h and 24 h, respectively, after induction of the RPAR. This time course appeared to correlate, with the influx of mononuclear inflammatory cells, although the effect of plasma leakage on changes in exudate PLA SA could not be determined. Oral adminstration of antiinflammatory drugs signficantly inhibited pleural fluid exudation and inflammatory cell influx to varying degrees. However, whereas these drugs additionally reduced total exudate protein and PLA, they had no effect on the concentration of either parameter, implying that pleural PLA may be passively linked to fluid and cell movement. [ABSTRACT FROM AUTHOR] |
