| Autor: |
White, J., Zembryki, D. |
| Zdroj: |
Agents & Actions; Jun1989, Vol. 27 Issue 3/4, p410-413, 4p |
| Abstrakt: |
Pretreatment of rat peritoneal mast cells with either Staurosporine or an analog K-252a, lead to a dose-related inhibition of histamine release when stimulated with Anti-IgE (IC: Staurosporine=110 n M; K-252a=100 n M). In contrast, the two PKC inhibitors (1-1000 n M) failed to inhibit histamine release induced by compound 48/80 (0.5-1 μg/ml). Exposure of Anti-Asc-IgE sensitized mouse bone marrow derived mast cells to Asc-BSA lead to the release of both histamine (510 ng±12.6 ng/10 cells) and immunoreactive Leukotriene C (27.0±12.6 ng/10 cells) LTC release was inhibited by Staurosporine and K-252a with an IC of 75 n M for both compounds. Pretreatment of rat peritoneal mast cells with PMA 100 n M lead to a small but significant release of histamine (18.3±3.6%). Pretreatment of these cells with K-252a or Staurosporine lead to a dose related inhibition of histamine release with an ED of 10 n M for Staurosporine and 60 n M for K-252a. Treatment of rat peritoneal mast cells with the calcium ionophore A23187 lead to a significant release of histamine which was not inhibited by either of the two kinase inhibitors (0.1-1000 n M). The two kinase inhibitors also inhibited mouse bone marrow derived mast cell proliferation in response to IL-3 with IC of 80 n M for Staurosporine and 270 n M for K-252a. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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