| Autor: |
Albinus, M., Armbruckner, L., Klein, S., Maier, R. |
| Zdroj: |
Agents & Actions; Apr1989, Vol. 27 Issue 1/2, p173-176, 4p |
| Abstrakt: |
Guinea-pig oxyntic cell tubulin has been isolated and in vitro aggregation has been studied. The spontaneous assembly of isolated tubulin was significantly accelerated and increased bt 1 mmol/l GTP. Histamine and forskolin increased tubulin polymerization only when detergent dispersed oxyntic cells or crude membranes were added. The forskolin response occurred very rapidly with an EC of approximately 30 μmol/l and did not require GTP. Histamine promoted tubulin aggregation with an EC of about 5 μmol/l only in the presence of GTP. Ranitidine completely inhibited the effects of histamine. From these data it is suggested that, in the oxyntic cell, histamine H-receptor activated adenylate cyclase and the corresponding increase in cAMP play a role in eliciting characteristic ultrastructural changes by initiating formation of microtubules as a first step in the cascade of events leading to an increase in the secretory surface area. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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