| Autor: |
Cheret, Anne-Marie, Laboisse, Christian, Roumagnac, Isabelle, Augeron, Chantal, Lewin, Miguel |
| Zdroj: |
Agents & Actions; Mar1986, Vol. 17 Issue 5/6, p436-440, 5p |
| Abstrakt: |
The human gastric epithelial cell line HGT-1 possesses adenylate cyclase-coupled histamine H receptors. To test the cellular homogeneity or heterogeneity with respect to these receptors, we have isolated 7 clones from the HGT-1 line and studied their basal and histamine-stimulated adenylate cyclase activities. Basal adenylate cyclase activities of the clones did not differ significantly, nor did 10 m M NaF-nor 0.1 m M Gpp(NH)p-stimulated activities. However, histamine stimulation of adenylate cyclase varied among clones from 1.9 fold to 5.4 fold basal activity. The EC50 values, determined in 3 clones, were not significantly different. These findings support the heterogeneity of histamine responsiveness of the human gastric cell line HGT-1. In addition, they suggest that highly histamine-responsive clones may be useful models to study the gastric histamine H-receptor and its specific antagonists in the human. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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