Isolation and characterization of valine-resistant mutants of Nicotiana plumbaginifolia.

Autor: Marion-Poll, A., Missonier, C., Goujaud, J., Caboche, M.
Zdroj: Theoretical & Applied Genetics; Feb1988, Vol. 75 Issue 2, p272-277, 6p
Abstrakt: Haploid mesophyll protoplasts of Nicotiana plumbaginifolia were mutagenized by UV-irradiation. Protoplast-derived colonies were then selected for valine resistance on a medium containing 5 or 10 mM valine. From the resistant calli, plants were regenerated. Resistance was inherited as a recessive Mendelian character in seven clones. Mutations conferring valine resistance were shown to be allelic. Protoplast-derived cells of L-valine-resistant plants were also resistant to L-threonine. Resistance to valine was based on a reduced valine uptake rate. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index