Isolation of a new restriction enzyme, ApaCI, an isoschizomer of BamHI produced by Acetobacter pasteurianus.
| Autor: | Grones, J., Turňa, J. |
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| Zdroj: | Folia Microbiologica; Oct1992, Vol. 37 Issue 5, p353-356, 4p |
| Abstrakt: | A new Type II restriction endonuclease ApaCI purified from Acetobacter pasteurianus is an isoschizomer of BamHI that cleaves at the nucleotide sequence 5′-G/GATCC-3′ of double-stranded DNA. The single restriction activity present in this strain permits rapidly purified 30 000 units of cleavage activity from 10 g of freshly harvested cells. The resulting ApaCI preparation is free of contaminant nuclease activities that might interfere with in vitro manipulation of DNA. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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