Establishment and characterization of a cell line of congenital primitive neuroectodermal tumor of soft tissue.

Autor: Fukushige, Takahiko, Nagoshi, Makoto, Hachitanda, Yoichi, Ueno, Takaki, Zaizen, Yoshio, Suita, Sachiyo, Tsuneyoshi, Masazumi, Kaneko, Yasuhiko, Nakagawara, Akira
Zdroj: Virchows Archiv B Cell Pathology; Dec1992, Vol. 62 Issue 1, p159-166, 8p
Abstrakt: A new human cell line, termed Muraoka, has been established from the recurrent tumor of a case of congenital primitive neuroectodermal tumor (PNET) arising at the temporofacial region of a male infant. The microscopic findings of this cell line were epithelioid, and the xenografted tumor in a nude mouse consisted of the malignant epithelioid cells. Immunohistochemically, the cells were positive for neuron-specific enolase, S-100 protein, carcinoembryonic antigen, cytokeratin, epithelial membrane antigen, and glial fibrillary acidic protein. These findings were quite smiliar to those of the epithelioid cells in the original tumor and of the xenografted tumor cells. Neither chromosomal abnormalities nor N-myc amplification were observed. Morphological differentiation after treatment with N-2′-Odibutyryladenosine 3′:5′-cyclic monophosphate (BtcAMP), all-trans-retinoic acid (RA), prostaglandin E (PGE, and 5-bromo-2′-deoxyuridine (BrdU) showed two different results. Bt-cAMP and PGE induced neuronal differentiation with the extension of neurites, whereas RA and BrdU predominantly induced Schwannian differentiation (flat cells). In these respects, the cell line Muraoka seems to be useful for studying characteristics of PNET as well as for developing the new treatments against such tumors. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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