| Autor: |
Wiedmer-Bridel, Jacqueline, Vogel, A., Hedinger, Chr |
| Zdroj: |
Virchows Archiv B Cell Pathology; Jun1978, Vol. 27 Issue 1, p267-277, 11p |
| Abstrakt: |
Histological examination of numerous biopsies from mature testes, repeatedly showed irregularities of the elastin staining in the tunica propria of the seminiferous tubules. Even when abundant elastic fibers were visible by light microscopy, no elastic fibers were demonstrable in the electron microscope in ultrathin sections of testicular tubules embedded in Epon and contrasted with phosphotungstic acid (PTA). In 43 biopsies from 22 men aged 17-39 years (19 investigations of sterility and three patients with hypogonadotropic hypogonadism) we therefore checked for the occurrence of elastic fibers in the wall of the seminiferous tubules. Matrix loci of elastic fibers could indeed be demonstrated by electron microscopy using PTA and potassium permanganate (KMnO), but only after embedding in araldite. Under these conditions, light and electron microscopic findings agreed with each other. The appearances of moderate and severe testicular tubular atrophy differed slightly from one another with regard to the amount of elastin. In the 'Sertoli cells only syndrome', elastic fibers were demonstrable only outside the hyalinized inner layer. In the Klinefelter syndrome, only 'uncertain' elastin loci were present, but greatly increased microfibrils were to be seen using the electron microscope. No elastic elements and only very sparse microfibrils were present in the tunica propria of the tubules of young men with hypogonadotropic hypogonadism. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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