| Autor: |
Kele, Péter, Illyés, Eszter, Mezö, Gábor, Dóda, Margit, Mák, Marianna, Kaposi, András D., Sebestyén, Ferenc |
| Zdroj: |
Letters in Peptide Science; Jul1999, Vol. 6 Issue 4, p235-238, 4p |
| Abstrakt: |
Fluorescent derivatives of bovine neurotensin 8-13 fragment were prepared. For N-terminal labelling, 4-[7-hydroxycoumaryl]acetic acid (Hca), 4-[7-methoxycoumaryl]acetic acid (Mca) and 2-amino-3-[4-[7-methoxycoumaryl]]propionic acid (Amp) were used while the C-terminus of the peptide chain was elongated with Amp. The fluorescence excitation and emission spectra of the peptide derivatives were studied. Hca- and Mca/Amp-derivatives were easily distinguishable because of the 60 nm shift of their emission maxima. Compared with the natural sequence, the presence of an N-terminal label did not influence the biological potency in a longitudinal muscle strip of guinea-pig ileum, while labelling at the C-terminus considerably reduced the activity of the peptide. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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