A novel method employing polymerase chain reaction to disrupt genes lacking convenient restriction enzyme sites in yeast.

Autor: Wu, Mian, Shang, Hui-Shen, Yeong, Ching-Yuen, Tan, Priscilla, Lu, Zhen-Xin, Wong, Sek-Man
Zdroj: Molecular Biotechnology; Feb1995, Vol. 3 Issue 1, p72-74, 3p
Abstrakt: A novel method employing polymerase chain reaction was developed for the disruption of yeast genes lacking convenient restriction enzyme sites. The method was found to be easy and effective. Using this method, a yeast YKE2 gene (a yeast homolog of murine k-region expressed genes) was successfully disrupted by replacement of HIS3 marker gene. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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