| Autor: |
Lawson, T.S., Connally, R.E., Vemulpad, S., Piper, J.A. |
| Předmět: |
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| Zdroj: |
Letters in Applied Microbiology; Mar2012, Vol. 54 Issue 3, p263-266, 4p, 1 Color Photograph, 1 Chart |
| Abstrakt: |
Aims: To test the feasibility of identifying Staphylococcus aureus with a fluorescence in situ hybridization (FISH) assay that uses a single hot-plate and urea-NaCl reagents. Methods and Results: Slides spotted with S. aureus and treated with methanol and lysozyme were incubated with urea-NaCl reagents on a hot-plate with a precise temperature control and identified with specific DNA probes. Conclusions: Staphylococcus aureus was detected and differentiated from Staphylococcus epidermidis in 1 h with a novel FISH method that used a single hot-plate and in the absence of dimethyl formamide. Significance and Impact of Study: A rapid hot-plate FISH assay with urea-NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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