| Autor: |
Blakeley, Stephen, Gottlob-McHugh, Sylvia, Wan, Jiangxin, Crews, Linda, Miki, Brian, Ko, Kenton, Dennis, David |
| Zdroj: |
Plant Molecular Biology; Jan1995, Vol. 27 Issue 1, p79-89, 11p |
| Abstrakt: |
Clones encoding two different forms of plastid pyruvate kinase (PK; EC 2.7.1.40) have been isolated from both castor and tobacco seed cDNA libraries. One form, designated PKA, from castor was described in a previous report, and the tobacco homologue of PKA has now been isolated. In addition, a second cDNA, designated PKG, has been identified and sequenced in both species. Western blot analysis, using antibodies raised against protein overexpressed from these clones, indicates that they encode the two predominant polypeptides of plastid pyruvate kinase from developing castor endosperm. In castor, both PKA and PKG are encoded by single genes. In the allotetraploid Nicotiana tabacum, there are two copies of each, one derived from each of the progenitors of this species. The expression of the genes for PKA and PKG was examined in various tissues from both castor and tobacco. In castor, both forms are expressed in developing and germinating endosperm and in the root but neither is expressed in the leaf. In tobacco, both forms are expressed in developing seeds but in mature tissues, PKA is most abundant in roots and PKG in leaves. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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