| Autor: |
Pavloff, Nadine, Biquard, Jean-Michel, Mariller, Marcel, Rabotti, Gian, Fossar, Nicole, Gay, Françoise, Semmel, Marianne |
| Zdroj: |
Molecular Biology Reports; Jun1987, Vol. 12 Issue 2, p127-131, 5p |
| Abstrakt: |
Activated CH-Sepharose 4B and protein A Sepharose CL-4B can bind, selectively and non-specifically, polypeptides from chick embryo cells. The major polypeptides bound have apparent molecular masses of 57-60 kDa and 47-49 kDa and cannot be eluted by extensive washing with buffers containing detergents. One of the 57-60 kDa polypeptides was identified by immunoblotting as the transforming protein of Rous Sarcoma Virus (RSV), pp60src. This polypeptide could be removed from the solid phase immunoabsorbant with 60% dimethylsulfoxide, but not with 2% SDS, 5% β-mercaptoethanol, 1 M NaCl or 0.1% Tween 20. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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