| Autor: |
Erickson, R., Ferrucci, S., Rahe, B., Rosenberg, M., Morello, D. |
| Zdroj: |
Molecular Biology Reports; Dec1984, Vol. 10 Issue 2, p109-113, 5p |
| Abstrakt: |
We have investigated differences in CpG methylation between F9 embryonal carcinoma cells in vitro and as tumor cells grown in vivo using Msp I and Hpa II restriction isoschizomers. Southerns were hybridized with two low copy number probes, mouse major β-globin (f7) and a class I, histocompatibility-2 cDNA clone (pH-2-4). In each case, the tumor-DNA was hypomethylated while the DNA from F9 cells grown in vitro was moderately methylated. We conclude that growth conditions or cell-cell interactions can greatly affect methylation of CpG sites. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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