In vitro cleavage of 45S pre-ribosomal RNA and of giant heterogeneous RNA extracted from human leukemic cells.

Autor: Torelli, U., Ferrari, St., Torelli, G., Cadossi, R., Ferrari, Se., Montagnani, G., Narni, F.
Zdroj: Molecular Biology Reports; Dec1977, Vol. 3 Issue 6, p403-411, 9p
Abstrakt: 45S ribosomal precursor RNA and large heterogeneous RNA molecules (>45S) extracted from human leukemic cells were incubated in vitro with purified RNase III, which specifically attacks double-helical RNA regions. About 50% of the ribosomal precursor was cleaved into two major fragments sedimenting at 28S and 32S respectively. A limited number of cleavages was also introduced in about 40% of heterogeneous RNA molecules sedimenting faster than 45S, causing a partial 'shift' to a polydisperse distribution in the 10S-45S range. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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