| Autor: |
Konings, Ruud, Schoenmakers, John |
| Zdroj: |
Molecular Biology Reports; Feb1974, Vol. 1 Issue 5, p251-256, 6p |
| Abstrakt: |
A method is presented which allows one to follow in a relative rapid and accurate way the synthesis of gene 5 protein of the bacteriophage M13 in a DNA-dependent cell-free system. Due to its unique property of binding selectively to single-stranded but not to double-stranded DNA, gene 5 protein can readily be separated, by means of sucrose densitygradient centrifugation, from the other polypeptides synthesized in the cell-free system. The meaning of this technique for the elucidation of the mechanism(s) which regulates gene 5 protein synthesis is discussed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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