| Autor: |
Thiele, Danielle, Sarocchi, Marie-Thérèse, Guschlbauer, Wilhelm, Lezius, Axel, Marck, Christian |
| Zdroj: |
Molecular Biology Reports; Oct1973, Vol. 1 Issue 3, p155-160, 6p |
| Abstrakt: |
The CD spectra of three polydeoxyribonucleotide complexes, poly(dG-C). poly(dG-C), poly(dA-G). poly(dT-C) and poly(dA-C). poly(dT-G) have been studied as a function of pH at 25°C. Only poly(dA-G). poly(dT-C) showed large hysteresis, analogous to that observed for poly(dG). poly(dC), while the alternating pur-p-pyr containing polymers reversible titration curves exhibited. The hysteretic form R of poly(dA-G). poly(dT-C) is metastable at neutrality and reverts spontaneously and irreversibly to the neutral from N upon heating to about 60°. A whole titration cycle to pH 2.5 and back to neutrality has to be performed in order to obtain the metastable form R again. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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