Regulation of the expression of lactate dehydrogenase isozymes in human lymphocytes.

Autor: Wollberg, Patrik, Dean Nelson, B.
Zdroj: Molecular & Cellular Biochemistry; Mar1992, Vol. 110 Issue 2, p161-164, 4p
Abstrakt: Mitogen activation of human peripheral lymphocytes leads to a switch in the isozymes of LDH; resting cells contain low activities of only the B and BA forms, whereas activated cells contain high activities of the A and AB forms. B LDH is not altered in activated cells. In this study we show that the appearance of the A subunits occurs concomitantly with a several fold increase in the steady state levels of LDH-A mRNA. Responses in LDH-A mRNA are observed within 12 hrs of activation, and are, thus, associated with the G0/G1 transition or with early G1 (Marjanovic et al. Exp. Cell Res. (1991) 193: 425-431). Maximal expression of LDH-A mRNA requires both phorbol ester and concanavalin A, implying a complex regulatory pathway involving cascade systems activated through both the antigen receptor (TR) and protein kinase C. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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