Growth-associated protein 43 is down-regulated in cultured astrocytes.

Autor: Vitković, Ljubiša, Mersel, Marcel
Zdroj: Metabolic Brain Disease; Mar1989, Vol. 4 Issue 1, p47-53, 7p
Abstrakt: Growth-associated protein 43 (GAP-43; other designations-pp46, F1, B-50, p-57) is an abundant, neural, membrane-associated protein involved in synaptic plasticity and regeneration. We recently reported that GAP-43 is present in plasma membranes of cultured rat astrocytes. In the present study the level of astrocytic GAP-43 was assessed by indirect immunofluorescence labeling of cells with a specific anti-GAP-43 serum and immunoblotting of plasma membrane proteins. The results indicate that all astrocytes from 1-day-old cortex contained GAP-43 and those differentiated in culture did not. Furthermore, GAP-43 decreased dramatically during 3 weeks in culture. These results are consistent with the developmental down-regulation of GAP-43 in vivo. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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