| Abstrakt: |
Transcription of the three unlinked, homologous STA1- 3 glucoamylase-encoding genes, involved in starch degradation by Saccharomyces cerevisiae, was previously shown to be down-regulated by the presence of STA10, acting via three upstream repression sequence regions that were identified in the STA2 promoter. Here we report the cloning and characterization of a putative transcriptional activator gene, MSS10 ( multicopy suppressor of S TA 10), which, when present in multiple copies, overcomes STA10 repression. Deletion of MSS10, located on chromosome XV, resulted in media-specific extinction of glucoamylase synthesis. The nucleotide sequence of MSS10 is identical to three other genes from S. cerevisiae identified as: FUP1, a gene that enhances iron-limited growth; PHD2, a gene identified for its ability to induce pseudohyphal growth in diploid cells grown on nitrogen-limited media; and MSN1, a gene encoding a transcriptional activator involved in invertase regulation. [ABSTRACT FROM AUTHOR] |
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