| Autor: |
Wright, Michel, Castillo, Lucas, Oustrin, Marie-Louise |
| Zdroj: |
Current Genetics; Apr1980, Vol. 1 Issue 3, p203-208, 6p |
| Abstrakt: |
A screening procedure for cell cycle mutants among is mutants of Physarum has been developed for unsynchronized microplasmodia. The synchronization of the microplasmodia and the ratio of pre- to post-mitotic nuclei were evaluated after a transient shift-up to the non-permissive temperature. In contrast with wild type and is mutants not blocked in a cell cycle event, putative cell cycle mutants were synchronized. Among them, three strains which showed a reduction of DNA synthesis had an abnormally increased ratio of pre- to post-mitotic nuclei. Thus, this methodology seems to be efficient and more rapid than the previously published procedures for detecting cell-cycle mutants of Physarum. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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