Two-compartment behavior during transport of folate compounds in L1210 cell plasma membrane vesicles.

Autor:	Yang, C., Dembo, M., Sirotnak, F., Yang, C H, Sirotnak, F M
Předmět:	FOLIC acid metabolism ANIMAL experimentation BIOLOGICAL models BIOLOGICAL transport CELL membranes CELL physiology COMPARATIVE studies DYNAMICS FOLINIC acid LEUKEMIA MATHEMATICS RESEARCH methodology MEDICAL cooperation METHOTREXATE MICE RESEARCH RESEARCH funding EVALUATION research
Zdroj:	Journal of Membrane Biology; 1982, Vol. 68 Issue 1, p19-28, 10p
Abstrakt:	The transport of [3H] 1,L 5-formyltetrahydrofolate, [3H] folic acid, and [3H]methotrexate by L1210 cell plasma membrane vesicles exhibited multicompartmental behavior. Two separate vesicular compartments (parallel relationship) of approximately equal volume were revealed during measurements of influx and efflux. Flux in one compartment was rapid, saturable, highly temperature-sensitive, and inhibited by pCMBS. Flux in the other compartment exhibited all of the characteristics of passive diffusion. These results imply that our plasma membrane vesicle preparations consist of a mixture of two functional species. Transport of folate into one of these species occurs by passive diffusion alone, whereas transport into the other kind of vesicle occurs by both passive diffusion and carrier-facilitated transport. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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