Abstrakt: |
Oxidation of toad urinary bladder epithelial cell membranes by periodate in the bathing medium altered vasopressin-stimulated transport of urea or of water and sodium depending on whether periodate bathed the mucosal or serosal surface. Subsequent reduction with tritiated borohydride was then used to label the oxidized cell membrane components. I found that: Thus, oxidation by periodate separates a vasopressin-stimulated transport system for urea from those for water and sodium, and allows labelling of several membrane glycoproteins which may be associated with the urea transport pathway. [ABSTRACT FROM AUTHOR] |