| Autor: |
Renneberg, J., Persson, K., Christensen, P. |
| Zdroj: |
European Journal of Clinical Microbiology & Infectious Diseases; Sep1990, Vol. 9 Issue 9, p659-663, 5p |
| Abstrakt: |
The antibody response in patients with Listeria monocytogenes septicemia and/or meningitis was investigated using Western blot analysis (WBA). Protein antigen preparations were produced from two strains of Listeria monocytogenes, representing serogroup 1 and 4, by sonication and differential centrifugation. IgG antibodies from 8 (50 %) of 16 patients with culture verified septicemia and/or meningitis due to Listeria monocytogenes reacted with a 93 kDa antigen from serogroup 1, in contrast to IgG antibodies from only 1 (2 %) of 51 controls; these controls represented 21 patients with infections caused by other bacteria and 30 apparently healthy blood donors. Furthermore, IgM antibodies from 3 (19 %) of the patients with listeric infections bound to a 106 kDa protein antigen in contrast to none of the controls. In 3 (33 %) of 9 patients from whom acute and convalescence serum were available, the patients responded by producing antibodies against new protein antigens. Current methods used in routine serological investigations, i.e. complement fixation and O-agglutination tests, were positive in only 4 (24 %) of the 16 patients with listeriosis, The results point to the possibility of designing new immunoassays for detection of septicemia and meningitis caused by Listeria monocytogenes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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