| Autor: |
Okunaka, Tetsuya, Aizawa, Katsuo, Kato, Harubumi, Konaka, Chimori, Kawabe, Hirofumi, Eckhauser, Marc, Bonaminio, Anthony, Hayata, Yoshihiro |
| Zdroj: |
Lasers in Medical Science; Mar1991, Vol. 6 Issue 1, p7-13, 7p |
| Abstrakt: |
An endoscopic laser fluorescence spectrophotometer has been developed to measure the fluorescence spectrum of haematoporphyrin derivative (HPD) in situ. In this study the demonstration of HPD accumulation in experimentally induced atheroma within the aortas of atherosclerotic rabbits is reported. Atheromas were induced in rabbit aortas after aortic intimai injury by balloon catheterization and hypercholesterolemic diets. Subsequently, an ultra-thin diagnostic angioscopic catheter was introduced into the descending aortas of these rabbits under anaesthesia, 24 h after the intravenous injection of 5 mg kg HPD. Characteristic red-shifted peaks of the fluorescence of HPD at 630 nm, 665 nm and 690 nm were detected in the fibrous plaques. In fatty streaks, however, the 630 nm and the 690 nm emission intensities were lower and the 665 nm peak was notably absent. Red-shifted HPD fluorescence was not detectable in normal areas of diseased aorta and in control animals. In addition, the emission spectra of HPD incubated with various lipid components representing the constituents of these atheromas were obtained. The resemblance of the emission spectrum of HPD incubated with sphingomyelin and cholesterol to that of the atheromatous lesions suggest that HPD may interact with these lipids in order to produce the red-shifted fluorescence maxima seen within atheromas. The possibility of future applications with this equipment for the diagnosis of atheroma is supported by this investigation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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